Introduction:
Trihydroxymethylaminomethane hydrochloride is referred to as Tris-HCl. Tris-HCl buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses.
Application:
Tris-HCl was used for protein crystal growth at different pH conditions. The low ionic strength of Tris-HCL buffer can be used for the formation of intermediate fibers of lamin in C.elegans. Tris-HCl is also one of the main components of the protein electrophoresis buffer. "In addition, Tris-HCL is an intermediate in the preparation of surfactants, vulcanization promoters, and some drugs." Tris-HCl was also used as a titration standard.
Buffer solution preparation:
Tris-HCl buffer solution with a concentration of 6.057g/L and a pH value of 8.8 was prepared by using Tris-hcl HCl as the solute and deionized water as the solvent.
Advantages:
1. Trihydroxymethyl amino methane hydrochloride has good water solubility, high solubility in water, and liquid clarity;
2. High purity, up to 99%, stable performance;
3. Do not precipitate with some metal ions (such as calcium and magnesium);
4. The ionic strength of Tris-HCl buffer is low;
5. Tris-HCl is more effective than the traditional phosphate buffer in the enzymatic assay.
Chemical properties:
White crystals.
Purpose:
1.The pH of all buffers was temperature and concentration dependent. For Tris buffer, pH increased by approximately 0.03 units per 1°C increase in temperature and decreased by 0.03 to 0.05 units per 10-fold dilution of pH.
2.For use in biological buffers
3.It is used as an organic synthesis intermediate
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