Product Description
Introduction:
4-hydroxyethyl piperazine ethosulfonic acid (HEPES) is an important hydrogen ion buffer, which has a good buffer ability in the pH range of 6.8 to 8.2, and can control a constant pH for a long time. Using a concentration of 10 ~ 50mmol/L, the general culture medium containing 20mmol/L 4-hydroxyethyl piperazine taurine can have a good buffer capacity, and has no toxic effect on cells.
Application:
HEPES (4-hydroxyethyl piperazinethesulfonic acid) is a non-ionic amphoteric buffer with high polarity, and is inert to a variety of chemical reagents and enzymes. It does not participate in and interfere with the process of biochemical reactions, and has no inhibitory effect on enzyme chemical reactions. Therefore, it can be used specifically for the research of proteins and enzymes that are highly volatile and ph-sensitive to organelles and very volatile.
Buffer solution preparation:
(1)hepes buffer can be directly added to the prepared culture medium according to the desired concentration, and then filtered to remove bacteria. 2.38 g HEPES was added to each 1000ml of culture medium, the pH was adjusted to 7.2 with lNNaOH after dissolution, and the bacteria were removed by filtration before use. At this time, the concentration of HEPES was 10mmol/L.
(2) 100x storage solution (lmol/L) could also be prepared. Before use, 99ml culture solution was added to lml storage solution, and the final concentration was 10mmol/L. lmol/L (100x) hepes buffer was prepared as follows: 23.8gHEPES was dissolved in 90ml double distilled water, the pH was adjusted to 7.5-8.0 with lNNaOH, and then the water was fixed to 100ml, filtered to remove bacteria, divided into vials (2ml/ bottle), and stored at 4ºC or -20ºC.
Purpose:
1.Biological buffer; Reaction buffer, prehybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear fraction; For RNA with T4RNA
2.Molecular biology grade is used for RNA3' -end labeling with T4 RNA ligase
3.Cell-cell adhesion, short-term cell aggregation culture, cleaning of tissue and cell buffer
Product Parameters
| Point of melting |
234-238 °C |
| Boiling point |
408ºC[at 101 325 Pa] |
| Density of density |
1.07 g/mL at 20 °C |
| Pressure of vapor |
0Pa at 25ºC |
| Index of refraction |
n20/D 1.339 |
| Storage conditions |
2-8°C |
| Degree of solubility |
H2O: 1 Mat 20 ° C, transparent, colorless |
| form |
White powder crystallization |
| Acid-base indicator discoloration ph range |
7.0 - 7.6 |
| PH value |
5.0-6.5 (1Maqueous solution) |
| Coefficient of acidity (pKa) |
7.5(at 25ºC) |
| Solubility of water |
Soluble |
| Maximum wavelength (λmax) |
λ: 260 nm Amax: 0.06λ: 280 nm Amax: 0.04 |
Certifications
Packaging & Shipping
Installation Instructions
Company Profile
Jiangsu Boquan Biotechnology Co., LTD. (hereinafter referred to as "Boquan") is a long-term engaged in fine chemical products and set production, sales and service in one of the company, the products are widely used in fine chemical raw materials, organic synthesis and pharmaceutical industry, chemical reagents, industrial sewage treatment, cosmetics, industrial electroplating, daily chemicals, food additives, pesticide chemical industry and other industries. Products are distributed all over the world, trusted by customers. The company's integrity, strength and product quality are widely recognized by the majority of customers and the industry. In terms of service, our company adheres to the principle of customer first, pursues the concept of "high quality, excellent price, and thoughtful service", and is committed to providing customers with quality products and pleasant shopping experience, no matter the size of customer demand, we can supply. In line with the principle of high quality, high efficiency and high service, we enjoy a good reputation in the same industry, and have a good reputation and influence, thanks to the support and love of manufacturers and customers.
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